95. Defective angiogenic markers and poor pregnancy outcomes in patients with hypertensive disorders of pregnancy: A pilot study in an Indian Population

Abstract

Objectives To assess angiogenic factors, matrixmetalloproteinases, and their inhibitors and cell adhesion molecules in maternal blood and placenta of various types of hypertensive disorders of pregnancy and to correlate with the pregnancy outcomes. Methods Plasma levels of soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1), soluble Endoglin (sENG), Transforming Growth factor beta (TGF-b), VEGF, Placental Growth Factor (PLGF), Matrixmetalloproteinases (MMPs), Tissue inhibitors of Matrixmetalloproteinases (TIMPs) and Cell adhesion molecules were analysed by ELISA kits in Gestational hypertension (GH), Late onset preeclampsia (LOPE), Early onset preeclampsia (EOPE), Eclampsia (E) and Control pregnant women (CPW) during third trimester. The mRNA/ protein expressions were assessed by RT-PCR/westernblot/IHCp respectively. The Pregnancy outcomes like the gestational age at the time of delivery (GA) and birthweight (BW) and APGAR score of the baby also were measured. Results The GA, BW, APGAR score of the baby were found to be significantly lower in EOPE and Eclampsia compared to CPW. The angiogenic factors, PLGF, TGF-b were significantly decreased in EOPE and Eclampsia compared to CPW, GH. Besides, the antiangiogenic factors sVEGFR1 and sEng were found to be elevated in HDP when compared to CPW. A significant correlation was observed between the angiogenic factors and the pregnancy outcome in HDP. Conclusions A defective angiogenic profile and poor birth outcomes were observed in Hypertensive disorders of pregnancy.