Abstract
This chapter explores the advantages and disadvantages of various inducible mammalian expression systems currently available that have many desired features. The chapter first gives a brief history of the original attempts at making inducible mammalian expression systems. Achieving tight regulation of expression in vitro and in vivo with a single promoter or control element has been a very difficult problem. The chapter presents a comparison among the three systems that have been characterized and made available commercially over the years. These three systems are the lacinducible expression system, the tetracycline-regulated expression system (which is available in different versions), and the most recent advancement in this field, the ecdysone-inducible expression system. The chapter reviews two systems based on prokaryotic elements from Escherichia coli: the lac-inducible system and the tetracycline-regulated system. Finally, the chapter evaluates the development of a new system based on regulatory elements from Drosophila melanogaster, the ecdysone-inducible system.
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