Abstract
A system has been established to study the 65Zn 2+ uptake into enzymatically active whole rat-liver nuclei in vitro. The incubation conditions have been elucidated and it appears that 37°, 20 min and a Zn 2+ nuclei ratio of 1 nmole Zn 2+ per 2.1 μg nuclear DNA are optimal. The rate of 65Zn 2+ taken up by the nuclei has been found to deviate in the presence of different bioligands such as amino acids or nucleic acid derivatives. A marked stimulation of 65Zn 2+ uptake can be observed with phosphate ions and nucleoside monophosphates. Ultrasonic treatment of nuclei suggests that most of the 65Zn 2+ being absorbed in vitro might be located in the nuclear membrane. If a 10-h pulse of 65Zn 2+ was applied to living rats a considerable amount of 65Zn 2+ could be detected in the soluble fraction containing the nucleoplasma.
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