Abstract
Conspicuous appearance of peroxisomes (microbodies) in yeast cells grown on n-alkanes or higher fatty acids has first been ascertained in our laboratory. Peroxisomes, isolated intact by differential and sucrose density gradient centrifugations, contain several enzymes participating in the alkane metabolism, such as long-chain alcohol dehydrogenase, long-chain aldehyde dehydrogenase, acyl-CoA synthetase, fatty acid β-oxidation system, catalase, NAD-linked glycerol-3-phosphate dehydrogenase, isocitrate lyase, malate synthase and carnitine acetyltransferase. The localization of these enzymes in peroxisomes indicates the indispensable roles of peroxisomes in the alkane metabolism by yeasts, that is, degradation of fatty acids derived from alkanes to C2-units via β-oxidation and synthesis of C4-compounds from C2-units through glyoxylate cycle. The roles of other cellular organelles, in addition to those of peroxisomes, in the alkane assimilation by yeasts are also discussed.
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