Abstract
To develop an efficient non-viral vector for gene delivery, we synthesized a new series of water-soluble polyamines containing hydroxyl, various amine, hydrophobic, and hydrophilic function groups. Transfection activity of these new polymers was evaluated in cell culture (murine melanoma BL-6 cells, human embryonic kidney 293 cells, Hep G2 cells and Hela cells) using CMV driven luciferase gene as a reporter. We demonstrate that the transfection activity of these new polymers was dependent on polymer structure. Among the four cell lines tested, poly-bis[2-hydroxy-3-(dodecylammonio)propyl]-ethyleneamines exhibit better transfection activity in Hep G2 and 293 cells. Poly-bis[2-hydroxy-3-(hexylammonio)propyl]-ethyleneamine were more active in Hep G2 cells. Poly-bis[2-hydroxy-3-(octadecylammonio)propyl]-ethyleneamines prefers 293 cells. A comprehensive study on activities of these polymers for airway gene delivery in mice will be presented.
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