533 マイクロ輸送デバイスの開発を目指したキネシン駆動微小管の運動解析アルゴリズムの構築(T10-3 マイクロ・ナノバイオテクノロジー(3):デバイス開発・医療応用を目指して,大会テーマセッション,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

Abstract

Microtubules (MTs) gliding on the kinesin-coated surface in vitro have a good feasibility for development of micro-scale transport systems. The key requirements in the development of the system include an image analysis to detect and recognize gliding MTs. In this study, we present an algorithm for detecting the position of the leading tips of gliding MTs. The algorithm operates on binarized fluorescent images of MTs and extracts the tips of MTs with a kernel. For verification, the algorithm was applied to a sample segment, and the distance between extracted coordinates with the algorithm and given coordinates were referred to an error. As a result, the algorithm caused the error, in which the measurement length was shorter than the actual length by only a half of the sample width. By applying the algorithm to the gliding MTs, the tips were well extracted following the trajectories of gliding MTs. The present algorithm for the tip-tracking may be useful for constructing nano-scale transport systems.