Abstract
Here, we describe the design of a novel mimic peroxidase, nanocomposites composed by 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) and cadmium sulfide (CdS). The H2TCPP–CdS nanocomposites can catalyze oxidation of substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2 and form a blue product which can be seen by the naked eye in 5min. The mechanism of the catalytic reaction originated from the generation of hydroxyl radical (·OH), which is a powerful oxidizing agent to oxidize TMB to produce a blue product. Then, we developed a colorimetric method that is highly sensitive and selective to detect glucose, combined with glucose oxidase (GOx). The proposed method allowed the detection of H2O2 concentration in the range of 4×10−6–1.4×10−5M and glucose in the range of 1.875×10−5–1×10−4M with detectable H2O2 concentration as low as 4.6×10−7M and glucose as low as 7.02×10−6M, respectively. The results provided the theoretical basis of practical application in glucose detecting and peroxidase mimetic enzymes.
Published Version
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