429 CONTRIBUTION OF BONE MARROW DERIVED STROMAL CELLS TO THE REGENERATION OF THE BLADDER AFTER PARTIAL OUTLET OBSTRUCTION

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology1 Apr 2011429 CONTRIBUTION OF BONE MARROW DERIVED STROMAL CELLS TO THE REGENERATION OF THE BLADDER AFTER PARTIAL OUTLET OBSTRUCTION Yukiko Kanno, Takahiko Mitsui, Hiroshi Sano, Kimihiko Moriya, Hiroshi Tanaka, and Katsuya Nonomura Yukiko KannoYukiko Kanno Sapporo, Japan More articles by this author , Takahiko MitsuiTakahiko Mitsui Sapporo, Japan More articles by this author , Hiroshi SanoHiroshi Sano Sapporo, Japan More articles by this author , Kimihiko MoriyaKimihiko Moriya Sapporo, Japan More articles by this author , Hiroshi TanakaHiroshi Tanaka Sapporo, Japan More articles by this author , and Katsuya NonomuraKatsuya Nonomura Sapporo, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.519AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In animal models of partial bladder outlet obstruction (PBOO), many experimental studies demonstrated ischemia and hypoxia induce significant bladder dysfunction. We previously revealed that the chemokine stromal cell-derived factor-1 induced by PBOO with ischemia/hypoxia is implicated in homing of bone marrow derived stem cells (BMCs) into the bladder. In the present study, we investigated the contributions of BMCs to the regeneration of the bladder with PBOO. METHODS The surgery of PBOO or Sham under anesthesia was performed at 6 weeks after allogenic bone marrow transplants from transgenic rats expressing green fluorescent protein (GFP) into lethally irradiated female Sprague-Dawley rats. The bladder was exposed on Week 6 and bladder tissue immunofluorescence was performed with antibodies against basement membrane marker (Laminin), urothelium maker (AE1/AE3), myofibroblast marker (Vimentin), smooth muscle marker (SMA), and GFP. RESULTS BMCs were accumulated more in the PBOO bladder compared to Sham. Most BMCs were accumulated around the basement membrane and lamina propria below the urothelium (Fig. A). BMCs were also found in the uroepithelium layer and some of those were double stained with GFP and AE1/AE3 (Fig. B). Some BMCs, which were located in the interstitial tissue, were double-stained with GFP and Vimentin. BMCs, which were migrated into the smooth muscle layer, showed fusiform morphologically and some of them were double-stained with GFP and SMA (Fig. C). CONCLUSIONS BMCs were homed into the PBOO bladder, and those cells have potential to differentiate into the several components of bladder tissue such as the uroepithelium cells, the myofibroblast cells, and smooth muscle cells in the bladder with PBOO. Thus, BMCs contribute to the regeneration of the PBOO bladder. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e173 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yukiko Kanno Sapporo, Japan More articles by this author Takahiko Mitsui Sapporo, Japan More articles by this author Hiroshi Sano Sapporo, Japan More articles by this author Kimihiko Moriya Sapporo, Japan More articles by this author Hiroshi Tanaka Sapporo, Japan More articles by this author Katsuya Nonomura Sapporo, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...