MP52-13 THE EFFECT OF PARTIAL BLADDER OUTLET OBSTRUCTION (PBOO) SEVERITY AND HYPERCHOLESTEROLEMIA ON ERECTILE FUNCTION IN RATS

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2015MP52-13 THE EFFECT OF PARTIAL BLADDER OUTLET OBSTRUCTION (PBOO) SEVERITY AND HYPERCHOLESTEROLEMIA ON ERECTILE FUNCTION IN RATS Serap Gur, Nur Bayatli, and Didem Yilmaz Serap GurSerap Gur More articles by this author , Nur BayatliNur Bayatli More articles by this author , and Didem YilmazDidem Yilmaz More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1730AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Erectile dysfunction (ED) and lower urinary tract symptoms (LUTS) caused by benign prostatic hyperplasia (BPH) are commonly coexist and very prevalent in men aged over 50 years. One of the leading theories of this interrelation is atherosclerosis-induced pelvic injury. We investigated the degree of partial bladder outlet obstruction (PBOO) induced by a ligation (with 5F or 4F catheter [c]) of the bladder neck and the role of atherosclerosis induced by hypercholesterolemic diet (HCD) on erectile function in rats. METHODS A total of 96 Sprague-Dawley male rats were divided into 6 groups: 1) Sham-operated; 2) PBOO (5Fc); 3) PBOO (4Fc); 4) HCD 5)HCD-PBOO (5Fc) and 6) HCD-PBOO (4Fc). HCD groups were fed 4% cholesterol and PBOO groups were subjected by ligation of the urethra for 6 wk. Erectile response was measured using cavernosal nerve stimulation by evaluating ratios of intracavernosal pressure (ICP)/mean arterial pressure. The relaxant and contractile properties of corpus cavernosum smooth muscle (CCSM) strips were evaluated in tissue baths. Immunohistochemical (IHC) staining for neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS), Rho-activated kinase (ROCK) and collagen deposition was performed. RESULTS The weight of bladder enhanced gradually in obstructed and combination groups and peaked in HCD-PBOO (4F)(Table). Relaxant and contractile responses of CCSM decreased depending on the severity of PBOO with HCD (Table). Rho-kinase inhibitor Y-27632 (10 μM) inhibited peak and sustained contractile responses to phenylephrine in groups (Table). We found lower nNOS, eNOS, higher Rho-kinase and collagen expressions in PBOO and combined groups. CONCLUSIONS The PBOO caused the more marked ED manifesting by considerable lowering of erectile response and the shift HCD in rats promoted. Our results suggest that the PBOO causes the functional and molecular changes, penile remodeling, accumulation of extracellular collagen in penile tissue, which may become more stressed with the HCD. The management of atherosclerosis may be beneficial in the treatment and continued research are needed for PBOO with HCD and will undoubtedly contribute to the future development of novel therapies for LUTS and ED. Sham-control PBOO(5F) PBOO(4F) HCD HCD-PBOO(5F) HCD- PBOO(4F) Bladder weight (g) 0.12 ± 0.01 0.25 ± 0.008*** 0.34 ± 0.004*** 0.53 ± 0.02*** 0.67 ± 0.004***### 0.91 ± 0.01 ***††† ICP/MAP (7.5V) 1.15 ± 0.06 0.66 ± 0.007*** 0.50 ± 0.020*** 0.52 ± 0.010*** 0.32 ± 0.01***### 0.10 ± 0.002***††† ACh (10-3M) 91.5 ± 1.5 78.9 ± 0.7*** 63.7 ± 2.5*** 46.7 ± 1.3*** 18.9 ± 1.1*** ### 6.2 ± 0.2*** ††† EFS relaxation (20Hz) 96.7 ±1.4 86.9 ± 1.6* 70.5 ± 2.1*** 62.6 ± 0.5*** 55.3 ± 1.9***### 16.6 ± 1.6***††† SNP (10-3M) 100 ± 0 100 ± 0 97.4 ± 0.9 91.4 ± 0.3 81.4 ± 0.3*** ### 66.1 ± 0.3*** ††† EFS contraction (40Hz) 69.5 ± 0.2 51.4 ± 0.8*** 43.8 ± 0.1*** 39.6 ± 0.02*** 29.0 ± 0.3***### 18.4 ± 0.9***††† Phe (10-3M) 90.9 ± 4.4 60.3 ± 2.6*** 43.5 ± 1.3*** 36.7 ± 0.8*** 28.1 ± 1.1***### 13.0 ± 1.1***††† Y-27 632(1μM) 100 ± 0 93.8 ± 0.3 43.5 ± 0.2*** 36.7 ± 0.5*** 28.13 ± 1.1***### 13.0 ± 0.8***††† Data are mean ± SEM (n=12-15). *p<0.05, **p<0.01, ***p<0.001 vs sham-control; ###p<0.001 vs PBOO (5F) and †††p<0.001 vs PBOO (4F). © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e632 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Serap Gur More articles by this author Nur Bayatli More articles by this author Didem Yilmaz More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...