1006 IMPAIRMENT OF SENSORY INNERVATION IN THE BLADDER NECK BY OUTLET OBSTRUCTION CONTRIBUTES TO THE DEVELOPMENT OF ERECTILE DYSFUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 20121006 IMPAIRMENT OF SENSORY INNERVATION IN THE BLADDER NECK BY OUTLET OBSTRUCTION CONTRIBUTES TO THE DEVELOPMENT OF ERECTILE DYSFUNCTION Qi Lei, Joseph Hypolite, Shaohua Chang, Robert Seftel, Samuel Chacko, Alan Wein, Allen Seftel, and Anna Malykhina Qi LeiQi Lei Glenolden, PA More articles by this author , Joseph HypoliteJoseph Hypolite Glenolden, PA More articles by this author , Shaohua ChangShaohua Chang Camden, NJ More articles by this author , Robert SeftelRobert Seftel Glenolden, PA More articles by this author , Samuel ChackoSamuel Chacko Glenolden, PA More articles by this author , Alan WeinAlan Wein Philadelphia, PA More articles by this author , Allen SeftelAllen Seftel Camden, NJ More articles by this author , and Anna MalykhinaAnna Malykhina Glenolden, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1108AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Benign prostatic hyperplasia (BPH) and erectile dysfunction (ED) are common problems in aging males worldwide. Clinical evidence of impaired sensation in ED suggests a neurogenic component of ED development, especially when ED is co-morbid with lower urinary tract symptoms. The objective of this work was to evaluate the role of the damage to bladder neck nerves during partial bladder outlet obstruction (PBOO) on sensory innervation of the corpus cavernosum (CC) and CC smooth muscle (CCSM) function using an animal model of PBOO. METHODS Four groups of adult male Sprague Dawley rats (N=32) were included in the study: sham operated (control), PBOO surgery, sham and PBOO groups with injected retrograde tracers. PBOO was surgically induced by a partial ligation of the bladder neck during laparotomy. Retrograde labeling technique was used to label L6-S2 dorsal root ganglion (DRG) neurons which innervate the urinary bladder and CC. The contractility and relaxation of the CC smooth muscle strips was studied in vitro in response to different stimuli. RESULTS Urinary bladders from animals with PBOO weighted 308.75±61.83 mg (N=13, 2 weeks PBOO) when compared to sham group (126.60±12.80 mg, N=13, p≤0.05). L6-S1 DRG ganglia were removed bilaterally and sectioned to count bladder and CC labeled sensory neurons. The obtained results showed that L6-S2 DRG ganglia receive input from both the urinary bladder and CC and 2-3% of all labeled neurons were double labeled from both organs suggesting some overlap in sensory innervation. The contractile response of CC muscle strips to electric stimulation was significantly reduced in PBOO group (2.223±0.3262 g/mg, p≤0.05) when compared to control (3.6367±0.3577 g/mg). Force generation of CCSM to 125 mM KCl was also significantly reduced in the PBOO group (2.61±0.35 g/mg vs 4.94±0.91 g/mg in control, p≤0.05). After CCSM was pre-contracted with phenylephrine, relaxation induced by nitroprusside was significantly diminished in PBOO group (2.76±0.29 g/mg vs 3.66±0.27 g/mg in the control, p≤0.05). There was also a significant decrease in n-NOS expression in PBOO animals when compared to the sham group (47%, N=4, p≤0.05). CONCLUSIONS PBOO leads to the impairment of bladder neck innervation followed by an increase in CCSM tone and a down-regulation of nNOS expression in the CC smooth muscle. These results provide evidence that nerve damage in PBOO may contribute to the development of ED causing secondary changes in the contraction/relaxation mechanisms of CCSM. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e409 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Qi Lei Glenolden, PA More articles by this author Joseph Hypolite Glenolden, PA More articles by this author Shaohua Chang Camden, NJ More articles by this author Robert Seftel Glenolden, PA More articles by this author Samuel Chacko Glenolden, PA More articles by this author Alan Wein Philadelphia, PA More articles by this author Allen Seftel Camden, NJ More articles by this author Anna Malykhina Glenolden, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...