Abstract
A chemical marker for poly-β-hydroxybutyrate (PHB), 2-butenoic acid, was identified in microbial pyrograms. The use of 2-butenoic acid as a chemical marker was validated by correlation with results from an extraction, hydrolysis, and derivatization GC/MS method for determination of PHB, by growth trials profiling PHB content as function of culture age, and by comparative pyrolysis studies of a diverse group of organisms. PHB was shown to be a common chemical present in microorganisms of the family Legionellaceae and high levels were not found in the family Enterobacteriaceae. In common with many other bacilli, B. anthracis was also found to contain PHB.
Published Version
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