Abstract
Abstract Background In the present study, we aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent. Results A total of 120 isolates were isolated from different soil samples and a total of 62 isolates showed positive results with Nile blue A staining, a specific dye for PHB granules and 27 isolates produced PHB using cardboard industry effluent. The selected isolate NA10 was identified as Bacillus sp. NA10 by studying its morphological, biochemical, and molecular characteristics. The growth pattern for the microorganism was studied by logistic model and exactly fitted in the model. A maximum cell dry weight (CDW) of 7.8 g l−1 with a PHB concentration of 5.202 g l−1 was obtained when batch cultivation was conducted at 37°C for 72 h, and the PHB content was up to 66.6% and productivity was 0.072 g l−1 h−1 in 2.0 L fermentor. Chemical characterization of the extracted PHB was done by H1NMR, Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), Gas chromatography–mass spectrometry (GC-MS) analysis to determine the structure, melting point, and molecular mass of the purified PHB. The polymer sheet of extracted polymer was prepared by blending the polymer with starch for packaging applications. Conclusions The isolate NA10 can be a good candidate for industrial production of PHB from cardboard industry waste water cost-effectively and ecofriendly.
Highlights
In the present study, we aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent
Teeka et al [29] used this method to screen the potential PHA-producing bacteria from soil, and Ramachandra and Abdullah [30] observed the colonies formed on nutrient-rich medium under ultraviolet light (UV) to screen for the pink fluorescence which indicated the presence of PHA producers
In this study, inexpensive cardboard industry waste water was tried as a carbon source to produce PHB
Summary
We aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent. PHA has been found useful as a new type of biofuel [8]. Besides all these properties and applications, wider use of PHAs is prevented mainly due to their high production cost compared with the oil-derived plastics [9]. Due to the large impact of the high price of carbon sources on production costs, one of the most important approaches to reduce costs is to use wastes and by-products as raw material for the fermentation process.
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