Abstract
Wide-field imaging facilitates the detection of rare events and reduces imaging time for large-area samples. Nano-scale imaging enables the detection and enumeration of individual nanoparticles and sub-cellular bioparticles. Uniting these two capabilities in a compact and field-portable on-chip imaging platform through lensfree holographic microscopy provides deep submicron resolution with high sensitivity over fields of view in the range 10–1800mm2 – approximately 150–2700 times larger than a typical 40×objective field of view. High resolution is achieved via a pixel super-resolution approach, while high sensitivity is obtained via a sample preparation procedure that generates self-assembled nanolenses around individual nanoparticles and viruses. The foundations of these computational imaging approaches, along with their methodology and results, are discussed.
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