Abstract
This paper reports on a novel micro imprinting process of collagen membrane by vacuum evaporation of hydrogel on a template under centrifugal force 〜500G. This process realizes 3D membrane micro/nano structures of extra cellular matrix polymer (e.g. collagen, elastin) with natural self-assembled fibrous structures kept as same as in vivo. The fabricated structure is highly suitable for cell culture scaffold in tissue engineering since no chemical cross-linking reagent is necessary in this process.
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