16S rRNA Gene Amplicon Sequence Data from Feces of Five Species of Wild Animals in Japan.

Kasumi Ishida-Kuroki,Ken Maeda,Tsutomu Sekizaki,Yoshihiro Nitta,Ai Takano,Hiroshi Shimoda,Nachiko Takeshita,Takehisa Chuma,Irene L G Newton

doi:10.1128/mra.00368-20

Abstract

We report 16S rRNA amplicon sequence data from feces from 58 wild boars, 60 feral raccoons, 9 wild Japanese badgers, 21 wild masked palm civets, and 8 wild raccoon dogs in Japan. The predominant bacterial taxa in the fecal microbiota were similar in part but varied among the animal species.

Highlights

Gut microbiota of animals are affected by host diet and phylogeny [1, 2]
The V3 to V4 regions of 16S rRNA genes in the extracted DNA were amplified with primers 341F (5=-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-NNNNN-CCTACGGGNGG CWGCAG-3=) and 805R (5=-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-NNNNN-GAC TACHVGGGTATCTAATCC-3=) [6], including an overhang adapter sequence (Illumina, San Diego, CA, USA)
Each operational taxonomic unit (OTU) was assigned at the family level against the Ribosomal Database Project (RDP) database [11] at 97% sequence identity

Summary

Introduction

Gut microbiota of animals are affected by host diet and phylogeny [1, 2]. Comparative analyses of microbiota in feces of domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa) have shown differences in the abundance of bacterial taxa [3]. Fifty-eight wild boars were killed by licensed hunters in Yamaguchi and Kagoshima prefectures in 2014 to 2019. Rectal feces from 60 feral raccoons, 9 wild Japanese badgers (Meles meles anakuma), 21 wild masked palm civets (Paguma larvata), and 8 wild raccoon dogs (Nyctereutes procyonoides), which were either trapped for control or died due to traffic accidents or fungal infection, were collected, as described above, by the Hikiiwa Park Center in Wakayama Prefecture in 2019.

Results

Conclusion