Abstract

Here, we report 16S rRNA amplicon sequence data from chicken cecal feces from Vietnam and Thailand. Lachnospiraceae, Ruminococcaceae, and Bacteroidaceae were dominant in cecal feces microbiota.

Highlights

  • We report 16S rRNA amplicon sequence data from chicken cecal feces from Vietnam and Thailand

  • The V3 and V4 regions of the 16S rRNA genes in the extracted DNA were amplified with S-D-Bact-0341-b-S-17 (5 ́-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGG GNGGCWGCAG-3 ́) and S-D-Bact-0785-a-A-21 (5 ́-GTCTCGTGGGCTCGGAGATGTGTATAA GAGACAGGACTACHVGGGTATCTAATCC-3 ́) primers (5) including an Illumina overhang adapter sequence (Illumina, San Diego, CA, USA)

  • Each operational taxonomic unit (OTU) was assigned at the family level against the Ribosomal Database Project (RDP) database (9) at 97% sequence identity

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Summary

Introduction

We report 16S rRNA amplicon sequence data from chicken cecal feces from Vietnam and Thailand. Cecal feces dropped on the floor were immediately collected using a 150-mm polypropylene spatula (As One, Osaka, Japan). Each sample was immersed independently in 500 ␮l of RNAlater stabilizing solution (Thermo Fisher Scientific, Waltham, MA, USA) in a conical tube.

Results
Conclusion
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