Abstract
Adrenal and liver tissues from a human fetus aborted after 6 months' gestation were sliced to 30μ thickness and incubated with dehydroepiandrosterone-4-14C in Krebs-Ringer bicarbonate buffer solution. 16αOH DHEA was found in the adrenal incubate after purification by alumina column chromatography, acetylation, thin layer chromatography and recrystallization to constant specific activity. The recrystallization data indicated that 4.9% of the radioactive material in the initial extract was 16αOH DHEA. However, the radioactive material in the androstenetriol fraction of the initial extract was not associated with carrier androstenetriol. Androstenetriol was detected in the liver incubate after purification by alumina column chromatography, two successive thin layer chromatographies and recrystallization to constant specific activity. The recrystallization data showed that 5.6% of the radioactive material in the initial extract was androstenetriol. But the radioactive material in the 16αOH DHEA fraction of the initial extract was dissociated from carrier 16αOH DHEA. The possible role of the fetus in the formation of estriol during pregnancy is discussed.
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