161 Single-cell RNA sequencing combined with interstitial fluid proteomics defines cell-type-specific immune gene regulation in atopic dermatitis

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Atopic dermatitis (AD) is the most common chronic inflammatory skin disease, but its complex pathogenesis is still only partly understood. To comprehensively characterize AD on both transcriptomic and proteomic levels in humans, we used skin suction blistering, a painless and non-scarring procedure that can simultaneously sample skin cells and interstitial fluid, and compared results to conventional biopsies. Suction blistering captured epidermal and most infiltrating immune cells equally well as biopsies, except for non-migratory CD163+ macrophages that were only present in biopsy isolates.