Abstract
Publisher Summary This chapter discusses methods for collection, transport, and storage of samples for hormone testing. The laboratory to which the specimens are to be sent should always be consulted as to their specific requirements for collection, storage, and transport of samples for particular tests. They will often also be able to supply requisition forms and special containers for transporting specimens. It should be ascertained whether the laboratory requires serum or plasma. Where there is a choice, plasma is frequently preferable because clotting releases proteolytic enzymes. For plasma, the recommended anticoagulant should be used. Heparin, for instance, interferes in some radioimmunoassay. Hemolysis should be avoided when taking the blood, as hemolysis may increase the rate of degradation of peptide hormones or interfere in the assay. Venous stasis should be minimized during blood collection as stasis will, at least in principle, raise the concentration of peptides, protein-bound hormones, and calcium.
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