1014 ADIPOSE DERIVED STEM CELLS AMELIORATE DIABETIC ERECTILE DYSFUNCTION IN THREE DIMENSIONAL CULTURE MODEL OF CAVERNOUS TISSUE

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You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (II)1 Apr 20131014 ADIPOSE DERIVED STEM CELLS AMELIORATE DIABETIC ERECTILE DYSFUNCTION IN THREE DIMENSIONAL CULTURE MODEL OF CAVERNOUS TISSUE Hazem Orabi and Tom F Lue Hazem OrabiHazem Orabi Assiut, Egypt More articles by this author and Tom F LueTom F Lue San Francisco, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.599AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Diabetes Mellitus (DM) is the most common single diagnosis associated with erectile dysfunction (ED). DM-associated ED continues to be difficult to treat as ED in diabetes results from combinations of derangement in each step in the process of penile erection. The aim of this study is to test if adipose derived stem cells in (ADSC) can ameliorate the pathological effects of DM on human erectile tissue in three dimensional in vitro co-culture model of cavernous tissue. METHODS Using biopsies of human penile tissues, human cavernous smooth muscle (HCSMC) and endothelial cells (ECs) were separately isolated and expanded. After cell phenotype verification, cavernous tissue was formed in vitro by seeding HCSMC followed by ECs. The cavernous tissue was subjected to a state of hyperglycemia and tissue was collected for histopathologic analysis for SMC, EC and collagen content. Apoptosis and expression of receptor of advanced glycation end products (RAGE) were also investigated. Allogenic human adipose stem cells (HACSC) were seeded into cavernous tissue on top of endothelial cells after induction of hyperglycemic condition. Cavernous tissue was detached and collected after 2 days. The effects of HADSC seeding were compared to those of euglycemic and hyperglycemic conditions. RESULTS The cavernous tissue was detached intact in consistent manner. The biochemical staining and IHC showed it is formed of only one layer of ECs and numerous layers of HCSMC separated by a collagen basement membrane. After the exposure to hyperglycemia, there was loss of ECs as shown by decreased expression of CD31, increase collagen content and apoptosis with increased expression of RAGE. Following the addition of HADSC, there was increased the expression of CD31 and stem cell differentiation into endothelial cells and smooth muscle cells. There was no effect on the expression of collagen IV. A decrease in cell apoptosis and RAGE expression were also noted. CONCLUSIONS ADSC can contribute to repair of endothelial damage and decrease apoptosis resulting from Diabetes Mellitus. ADSC have the ability to undergo differentiation toward ECs and SMC. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e416 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hazem Orabi Assiut, Egypt More articles by this author Tom F Lue San Francisco, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...