1127 HUMAN ADIPOSE TISSUE-DERIVED STEM CELLS DIFFERENTIATED INTO SMOOTH MUSCLE AND ENDOTHELIAL CELLS FOR TREATMENT OF ERECTILE DYSFUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research1 Apr 20111127 HUMAN ADIPOSE TISSUE-DERIVED STEM CELLS DIFFERENTIATED INTO SMOOTH MUSCLE AND ENDOTHELIAL CELLS FOR TREATMENT OF ERECTILE DYSFUNCTION Hazem Orabi and Tom Lue Hazem OrabiHazem Orabi San Francisco, CA More articles by this author and Tom LueTom Lue San Francisco, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.724AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Adipose tissue-derived stem cells (ADSCs), being easily, repeatedly and efficiently harvested with considerably less donor morbidity, represent ideal source for cellular treatment for erectile dysfunction. The aims of this study were to investigate if human adipose derived stem cells can be differentiated into smooth muscle cells (SMC) and endothelial cells (EC) and if these differentiated cells can be constructed into cell sheets and cavernous tissue. METHODS Human ADSCs were isolated and expanded. They were induced into SMC and EC using human recombinant TGF-β1 and endothelial growth medium-2 (EGM-2) respectively. The growth and morphology of the cells were followed up for 4 weeks. The phenotype of induced cells were checked for SMC markers; actin, calponin and heavy chain myosin and for endothelial cell markers; CD31 and Von Willebrand factor (vWF) through immunocytochemistry and western blot. Then, the cells were used to construct SMC and EC sheets by culturing them in thermosensitive dishes for 7–10 days. Confluent cultured cells were harvested as a contiguous cell sheet only by lowering temperature. The cell sheets were stained with H&E and Masson Trichrome and verified for smooth muscle markers and for endothelial cell markers. The formation of extracellular matrix of the both cell sheets was tested using Picrosirius Red staining and collagen IV. Both smooth muscle and endothelial sheets were layered into 3D cell-dense tissues and cultured in vitro for 7 days. RESULTS The induced cells showed positive staining for smooth muscle markers and endothelial cell markers after 3 weeks for SMC cells and 2 weeks for EC. The western blot confirmed their phenotype conversion. The cells sheets could be detached easily by lowering the temperature in intact form and viable condition. The sheets were formed of 2–5 cell layers that showed positive staining for smooth muscle and endothelial cell markers. They also showed positive staining for Picrosirius Red and collagen IV indicating the formation of extracellular matrix. Cell viability was demonstrated for the monolayer and bilayered cell sheets. CONCLUSIONS The results showed the ability of human ADSCS to form SMC and EC and constitute renewable source for cellular therapy. The cell sheets made of ADSCs form new technology for improvement of erectile dysfunction. The smooth muscle and endothelial sheets can used to form cavernous tissue in vitro to be delivered to penis. In vivo studies are our next step to verify the therapeutic potential of this new treatment modality. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e453 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hazem Orabi San Francisco, CA More articles by this author Tom Lue San Francisco, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...