Engineered small diameter vascular grafts by cell sheet engineering with human umbilical cord vein perivascular cells

Abstract

Event Abstract Back to Event Engineered small diameter vascular grafts by cell sheet engineering with human umbilical cord vein perivascular cells Beyza Gökçinar-Yagci1, 2 and Betül Çelebi-Saltik1, 2 1 Hacettepe University, Department of Stem Cell Sciences,Graduate School of Health Sciences, Türkiye 2 Hacettepe University, Center for Stem Cell Research and Development, Türkiye Pericytes are perivascular cells that surround endothelial cells of capillaries, microvessels and larger vessels; arteries and veins. They have important roles in vascular development (vasculogenesis and angiogenesis), stability, maturation and remodeling, regulation of vessel diameter and blood flow, blood pressure control, contractility and tone of vascular smooth muscle cells. The cell sheet engineering is based on the capability of cells to secrete and organize their own extracellular matrix (ECM). In this study our aim is to isolate human umbilical cord vein pericytes (CD146+ cells) then differentiate into smooth muscle cells and fibroblasts that will be used for generating tissue engineering vascular grafts and also reduce the time required to produce a tissue-engineered vascular graft. Human perivascular cells were isolated from umbilical cord vein and pericytes were purified by using MACs cell separation with CD146 microbeads. Perivascular cells were cultured in FGM-2, SMCGM-2 and EGM-2 medium for 21 days to investigate fibroblast, smooth muscle cell and endothelial cell differentiation. Cell differentiation was confirmed by immunofluorescence staining and flow cytometry analysis (fibroblast markers; Tenascin-C and Collagen type I, smooth muscle cell markers; Caldesmon and alpha smooth muscle actin, endothelial cell markers; VEGFR1, VEGFR2 and CD31). Next, a tissue-engineered vascular graft was fabricated by rolling the perivascular cells derived endothelial cell, smooth muscle cell and fibroblast sheets (Rapid cell sheet detachment from Poly(N-isopropylacrylamide)) around a mandrel. Different human ECM molecules such as collagen type I and IV, fibrin, elastin, fibronectin and glycosaminoglycans were used among the vascular layers to increase the mechanical properties of the graft. The results indicated that perivascular cells could differentiate into fibroblast, smooth muscle, endothelial cells and formed tubular vascular graft with ECM proteins. As a conclusion, differentiated pericytes offer an alternative cell source for constructing tissue engineered vascular graft. The authors wish to thank The Scientific and Technological Research Council of Turkey (Project Number: 113S815) for their financial support. The Scientific and Technological Research Council of Turkey (Project Number: 113S815) Keywords: Extracellular Matrix, Regenerative Medicine, 3D scaffold, tissue niche Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in constructing tissue substitutes Citation: Gökçinar-Yagci B and Çelebi-Saltik B (2016). Engineered small diameter vascular grafts by cell sheet engineering with human umbilical cord vein perivascular cells. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01061 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Beyza Gökçinar-Yagci Betül Çelebi-Saltik Google Beyza Gökçinar-Yagci Betül Çelebi-Saltik Google Scholar Beyza Gökçinar-Yagci Betül Çelebi-Saltik PubMed Beyza Gökçinar-Yagci Betül Çelebi-Saltik Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.