Pericyte-based collagen scaffold for vascular tissue engineering

Abstract

Event Abstract Back to Event Pericyte-based collagen scaffold for vascular tissue engineering Betül Çelebi-Saltik1, 2 and Beyza Gökçinar-Yagci1, 2 1 Hacettepe University, Department of Stem Cell Sciences, Graduate School of Health Sciences, Türkiye 2 Hacettepe University, Center for Stem Cell Research and Development, Türkiye Pericytes are a perivascular cell population embedded on the capillary wall in a shared basement membrane with the endothelium. During vasculogenesis and angiogenesis, endothelial cells secrete and/or present cell bound molecular cues that stimulate pericytes to proliferate, migrate and attach to nascent capillary tubes. It has been previously developed cell sheet engineering using temperature-responsive culture dishes in order to avoid traditional tissue engineering approaches. In this study our aim is to isolate human umbilical cord vein pericytes then differentiate into smooth muscle cells and fibroblasts then co-culture these cells on 3D collagen scaffold that will be used for generating tissue engineering vascular grafts. Pericytes were enzymatically isolated from human umbilical cord vein and were purified by using MACs cell separation with CD146 microbeads. They were cultured in fibroblast growth medium and smooth muscle growth medium for 21 days on poly(N-isopropylacrylamide) to obtain cell sheets. Cell differentiation was confirmed by immunofluorescence staining and flow cytometry analysis (fibroblast markers; Tenascin-C and Collagen type I, smooth muscle cell markers; Caldesmon, Calponin and Alpha smooth muscle actin). Collagen gel was prepared with human collagen type I (3mg/mL) + 10X DMEM (adjustment pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH). Collagen gel was covered by cell sheets (sandwich system) and vascular graft was incubated in 37oC for 7 days (Figure 1). The results indicated that perivascular cells could differentiate into fibroblast and smooth muscle and formed vascular graft with collagen gel. As a conclusion, differentiated pericytes offer an alternative cell source for constructing tissue engineered vascular graft. The authors wish to thank The Scientific and Technological Research Council of Turkey (Project Number: 113S815) for their financial support. The Scientific and Technological Research Council of Turkey (Project Number: 113S815) Keywords: Tissue Engineering, cell, 3D scaffold, tissue niche Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in mesenchymal and hematopoietic stem cell biology Citation: Çelebi-Saltik B and Gökçinar-Yagci B (2016). Pericyte-based collagen scaffold for vascular tissue engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02836 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Betül Çelebi-Saltik Beyza Gökçinar-Yagci Google Betül Çelebi-Saltik Beyza Gökçinar-Yagci Google Scholar Betül Çelebi-Saltik Beyza Gökçinar-Yagci PubMed Betül Çelebi-Saltik Beyza Gökçinar-Yagci Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.