066 In vivo tracking of antigen-specific skin-resident memory CD4+ T cells

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While development of skin-resident memory CD4+ T (TRM) cells homing to infected or inflamed skin has been well-characterized, in vivo tracking of antigen-specific CD4+ T cells in these processes remain undefined. In this study, we adoptively transferred both DNFB sensitized (Red) and OVA sensitized (Green) CD4+ T cells to naïve recipients, and challenged with DNFB and OVA, respectively, into the recipients’ ears. Then, we performed two photon intravital imaging to monitor the in vivo tracking of antigen-specific CD4+ T cells during development of skin TRM cells in our allergen-induced mouse model.