구강 질환 유발균인 Streptococcus mutans와 Porphyromonas gingivalis에 대한 모링가(Moringa oleifera) 잎의 항균 효과

Abstract

This study examined the antibacterial activity of Moringa oleifera ethanol extract (MOEE) against oral pathogens, Streptococcus mutans and Porphyromonas gingivalis. The antibacterial activity of MOEE was analyzed using a disk diffusion assay, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), and growth inhibition assay, and the inhibitory effect of nitric oxide synthesis and cytotoxicity was assessed using the Griess reaction and WST-1 assay in RAW 264.7 cells. NO production by LPS stimulation was inhibited dose-dependently without cytotoxicity. MOEE potently inhibited the disk diffusion of P. gingivalis than S. mutans, which was 19.72 mm and 17.22 mm at 300 mg/mL, respectively. In addition, the MIC of S. mutans and P. gingivalis was 0.8 mg/mL and 0.4~0.8 mg/mL, and the MBC of both pathogens was 0.8 mg/mL for both. MOEE also potently inhibited the growth of P. gingivalis and S. mutans, which was OD600 of 0.8 mg/mL MOEE-treated BHI broth was 0.219 and 0.125, respectively. Consequently, MOEE exhibited potent antibacterial activity, which was stronger against P. gingivalis than S. mutans. MOEE might be used as a functional material for preventing dental caries and periodontal diseases when the inhibitory activity of the biofilm and extracts from various solvents will be conducted in future studies.