Abstract
Purpose : To investigate the therapeutic effect of Lianbeijuqin (LBJQ) decoction on periodontitis in rats in vivo and in vitro. Methods : High performance liquid chromatography (HPLC) was used to analyze the main constituents of LBJQ. Periodontitis in rats was induced in rats by ligation with bacteria-infected silk. Gingival index (GI) and attachment loss (AL) were evaluated. Additionally, the antibacterial activity of LBJQ against Porphyromonas gingivalis, Prevotella intermedius, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Streptococcus sanguis and Streptococcus mutans were evaluated using minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values. Furthermore, the inhibitory effect of LBJQ on the release of TNF-α, IL-1 β and IL-6 was also determined. Results : HPLC revealed the presence of p hellodendrine, baicalin, palmatine, berberine, baicalein, wogonin and obacunone in LBJQ. LBJQ decoction dose-dependently (200, 400 and 800 mg/kg) and significantly decreased GI and AL (p < 0.05) in the periodontitis model rats. It also exerted significant antibacterial effect against P. gingivalis (MIC, 0.64 mg/mL; MBC, 2.56 mg/mL), P. intermedius (MIC, 0.32 mg/m; MBC, 2.56 mg/mL), A. actinomycetemcomitans (MIC, 1.28 mg/mL; MBC, 5.12 mg/mL), F. nucleatum (MIC, 0.16 mg/mL; MBC, 0.64 mg/mL), S. sanguis (MIC, 0.64 mg/mL; MBC, 5.12 mg/mL), and S. mutans (MIC, 0.16 mg/mL; MBC, 1.28 mg/mL). Moreover, LBJQ decoction dose-dependently and significantly decreased the release of TNF-α (p < 0.01), IL-1 β (p < 0.01) and IL-6 (p < 0.01) in LPSinduced RAW 264.7 cells. Conclusion : LBJQ exerts therapeutic activity against periodontitis in rats via inhibition of the release of inflammatory cytokines and growth of oral anaerobic bacteria. Keywords : Lianbeijuqin decoction, Periodontitis, Phellodendrine, Baicalin, Palmatine, Berberine, Porphyromonas gingivalis, Prevotella intermedius, Inflammatory cytokines
Highlights
Periodontitis is a chronic oral inflammatory disorder that occurs in tooth-supporting tissues such as gingiva and radices dentis
High performance liquid chromatography (HPLC) was used for the qualitative analysis of the constituents of LBJQ
The results of this study demonstrate for the first time, that LBJQ has potential therapeutic effect against experimental periodontitis in rats
Summary
Periodontitis is a chronic oral inflammatory disorder that occurs in tooth-supporting tissues such as gingiva and radices dentis. LBJQ is a traditionally used Chinese formula for periodontitis treatment, its therapeutic effects and pharmacological mechanisms of action are still not clear. The periodontitis model rats were divided randomly into 4 for groups (n = 10): control and 3 LBJQ treatments groups (200, 400 and 800 mg/kg). Another set of 10 rats were treated as normal group. In the evaluation of MBC, 0.01mL bacterial culture medium after the MIC was inoculated on an agar plates for growth another 24 h at 37 °C, and MBC is the lowest concentration of LBJQ without visible bacterial growth. Differences in means between two groups were analyzed with twotailed Student’s t-test and were considered statistically significant at p < 0.05
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