Abstract
Saccharomyces cerevisiae is not only an excellent source of B-complex vitamins but also a source of functional food as probiotics or prebiotics. Colorectal cancer is major cause of death in Taiwan. Approximately 70% of colorectal cancer is related to diets. Probiotics have positive health effects such as antioxidative, antitumor and antimutagenic activity. The objective of this study was to investigate effects of several S. cerevisiae cell fractions on the proliferation of human colon cancer cell, HT29, and the prevention of 4NQO-induced DNA damage on Int-407 cells. The possible anticytotoxic mechanisms were investigated by section-preincubation test. Comet assay explored the effects of yeast cell fraction against 4NQO-induced genetoxicity. The result revealed that heat-killed cells and crude cell walls exhibited the antiproliferation activity on HT-29 cells.The antiproliferation rate was 24.5%-52.83%. It was noted that heat-killed cells and crude cell walls reduced the cytotoxicity of 4-NQO against Int-407 showing the anticytotoxic activity. The viability of Int-407 increased from 49.7%-54.6% to 73.3%-82.2%. Heat-killed cells and crude cell walls also significantly reduced the 4NQO-induced genetoxicity. The scores of DNA damage decreased from 134.0-146.7 to 79.0-90.0 and 76.0- 93.3. It was found that heat-killed cells and crude cell walls might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells. Additionally, heat-killed cells might exert descytotoxic effects by bioremediation, thus reducing cytotoxicity of 4-NQO.
Published Version
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