Влияние агониста бета3-адренорецепторов мирабегрона на активность нейтрофилов венозной крови небеременных женщин

Abstract

The aim of this paper was to determine the effect of the beta3-adrenergic receptor agonist mirabegron on the free-radical activity of venous blood neutrophils in non-pregnant women. Materials and methods. Using the chemiluminescent method (BChL-07 biochemiluminometer), the parameters of the radical response of venous blood neutrophils in 40 non-pregnant women were determined in different phases of the cycle and on the first day after childbirth with additional cell stimulation with latex particles (0.08 μm). When the noise level was automatically subtracted, the following were recorded: maximum intensity of the synthesis of reactive oxygen species, time to reach the maximum of synthesis intensity, and area under the chemiluminogram curve, reflecting the total synthesis of radical particles over the course of 30 min. These parameters were determined before and after an in vitro introduction of the beta -adrenergic receptor agonist mirabegron (10–6 g/l), beta-adrenergic receptor blocker propranolol hydrochloride (10–6 g/l) and agonist against the background of the blocker into the venous blood. Results. Using the Wilcoxon signed-rank test, we showed an increase in the free-radical activity of neutrophils in women in the follicular phase of the menstrual cycle under the influence of mirabegron for all three evaluated parameters. In the luteal phase, mirabegron significantly increased the rate of reaction development and the total synthesis of radical particles, but did not affect the intensity of production of activated oxygen species. However, no effect was observed on the first day after delivery. The possible mechanism of the revealed mirabegron action is discussed in the article. The authors believe that changes in the activity of venous blood neutrophils under the action of mirabegron may be due to changes in the quantity or sensitivity of beta3-adrenergic receptors in different phases of reproduction.