Вміст неетерифікованих жирних кислот у плазмі крові кролів за гострого аргінінового панкреатиту та його корекції

Abstract

The aim of this work was to study the content of non-esterified fatty acids in plasma and liver of rabbits with acute arginine pancreatitis and its correction by linseed oil. The experiment was carried out on male rabbits breed gray giant with live weight 3.8–4.0 kg. The material for the study was sampled from blood and liver. Lipids from plasma and liver were extracted with a mixture of chloroform and methanol. After that the non-esterified fatty acids were isolated and methylated. Methyl esters of fatty acids were studied by the gas-liquid chromatography with the chromatograph ''Chrom 5'' (Prague,CzechRepublic). We have found that the content of non-esterified fatty acids decreases in the blood plasma and liver of rabbits with the acute arginine pancreatitis. It takes place at the expense of a reduce of saturated fatty acids with odd and even number of carbon atoms in a chain, monounsaturated fatty acids of the families ω-7 and ω-9 and polyunsaturated fatty acids of the families ω-3 and ω-6. That may indicate a greater use of non-esterified fatty acids for energy metabolism and esterification of lipids. We suppose that this is a consequence of the probable increase in content of non-esterified and esterified cholesterol in the rabbits’ blood plasma. Those processes provoke the cholesterol deposits in blood vessels and therefore cardiovascular diseases. We tried to influence on the processes by addition of linseed oil to the rabbits diet. We have found that in the linseed oil-fed rabbits the content of non-esterified fatty acids decreases at the expense of saturated fatty acids with odd and even number of carbon atoms in a chain and monounsaturated fatty acids of the families ω-7 and ω-9 inblood plasma and liver of the rabbits with acute arginine pancreatitis. Furthermore the levels of non-esterified polyunsaturated fatty acids of ω-3 family increase in the rabbits’ plasma and liver. As this takes place the ratio of non-esterified polyunsaturated fatty acids of ω-3 family to non-esterified polyunsaturated fatty acids of ω-6 family increased. The increase of non-esterified linolenic acid content in the rabbits’ blood plasma is apparently a result of a greater intake of linseed oil with food. In turn, the greater intensity of linolenic acid transformation in long-chain and unsaturated derivatives caused the increase of non-esterified docosapentaenoic and docosahexaenoic acids levels. Therefore, feeding with linseed oil led to normalizations of both the effective use of non-esterified fatty acids for energy processes and the level of esterified cholesterol in the blood plasma of rabbits with acute arginine pancreatitis.