ИЗМЕНЕНИЕ УРОВНЯ БАКТЕРИАЛЬНОЙ, СВОБОДНОЙ И СУММАРНОЙ АТФ ПРИ ЗАБОЛЕВАНИИ КОРОВ СУБКЛИНИЧЕСКИМ МАСТИТОМ

Abstract

The work reflects the results of studies to determine the level of bacterial, free and total ATP in the secretion of the udders of patients with cow mastitis. The level of ATP-bioluminescence, including total, free and bacterial ATP, was determined using the HY-LiTE®2 (Merk, Germany) luminometer and special test systems. When studying the secretion of udder cows with patients with clinical purulent mastitis, it was not possible to determine the correlation between number of microorganisms and ATP values, since microorganisms were absent in 50% of samples. It was found that in the secretion of the udder of cows with a subclinical form of udder inflammation, the values of somatic cells were 2,5-3 times higher than those of healthy animals. With the increase in the number of somatic cells, total ATP, in contrast to free, increased. At the maximum level of somatic cells, ATP parameters differed 4-fold. It was noted that with the increase in the total ATP in the secretion of the udders of sick cows, the content of microorganisms was proportionally increased, while there was no correlation between number of microorganisms and the level of free ATP. It was determined that an elevated level of free ATP and number of microorganisms was observed in 14.3% of udder secretion samples at low values of somatic cells (at the level of indices in healthy animals). It was noted that with the increase of number of microorganisms, the values of ATP of bacteria increase.