Abstract
The article presents the results of studies of sterilized milk on the presence of residual (total and free) ATP, the possibility of using sterile milk containing a significant amount of free ATP, as a full-fledged substrate for the cultivation of E. coli. The level of ATP-bioluminescence was determined by HY-LiTE®2 (Merk, Germany) luminometer in accordance with the manufacturer's instructions using special test systems. It has been established that with the sterilization of milk, somatic cells are destroyed, and only free ATP remains. By the level of free ATP, you can indirectly judge the amount of somatic cells in raw prefabricated milk taken by dairies and, as a consequence, the quality of the milk being procured. It was determined that when bacteria were introduced into sterilized milk after 6 hours of incubation, the level of free ATP decreased 5 times, the parameters of bacterial ATP increased 4 times against the background of an increase in the level of bacteria by 2 times. At the same time, it was noted that in the meat infusion agar during this time interval, the ATP of bacteria increased only by 1,3 times, in physiological salinity the indices remained at the same level.
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