Імуногістохімічна характеристика субпопуляцій лімфоцитів селезінки голубів та курей

Abstract

<p>The article is devoted to the study of immunohistochemical features of the spleen of birds based on the detection and morphometric calculation of clusters of the T- and B-lymphocytes. For the research, the selection of the spleen is performed at the stage of morphofunctional maturity in clinically healthy sexually mature birds of both sexes in the ratio 1: 1: blue pigeons (age 10-14 months), chickens of domestic breeds of Poltava clay (age 19-20 weeks). The slices of material are fixed in 10-12% chilled solution of neutral formalin, embedded in paraffin. Histological sections made on a microtome MS-2 and have a thickness not exceeding 5 micrometres. The mouse monoclonal antibodies of the Danish firm DAKO are used to detect and study subpopulations of lymphocytes. Morphometric studies are performed under light microscopy (Microscope Biolam-Lomo and Micros MC-50). The microphotography of histological preparations was carried out with CAM V 200 video camera. It is mounted on a microscope Micros MC-50. Subpopulations of lymphocytes CD4 +, CD8 +, СD19 +, СD20 + were detected in the red and white pulp of the spleen of pigeons and chickens, they are situated singly and diffusely, more often formed chains or aggregations. The CD4 + cells in pigeons and chickens are dominated in lymphoid sheaths near the vessels, amounted the 56.63 ± 4.03 pieces per conditional unit of area and 85.71 ± 6.09 pieces per conditional unit of area respectively. The populations of CD8 + cells are also predominated in lymphoid sheaths near the vessels, but their number was smaller than the CD4+ population by 1.81 times in chickens and by 2.36 times in pigeons. In the red pulp, CD4 + cells are occupied 7.67% of the total population of the splenic cells in chickens and 5.99% in pigeons. The CD8 + cell in the red pulp are less concentrated: 2,8% and 4,45% respectively. The index of immunoregulation of the spleen of chickens was 1.85, and the pigeons – 2.0. B-lymphocytes (cluster CD19 +, CD20 +) are concentrated in the B-dependent zone of the spleen – the lymphoid nodules. Most of the CD20 + cells are in lymphoid nodules of chickens (77.27%), in pigeons this indicator is 61.66%. Cells of CD19 + in lymphoid nodes are occupied approximately the same relative square of pigeons and chickens from their total amount in the pulp – 74.14% and 76.60%. The total number of T-lymphocytes (the population of CD4 +, CD8 +) are prevailed almost by two times in chickens, B-lymphocytes (populations CD19 +, CD20 +) in pigeons – by 1,6 times. In the spleen of pigeons and chickens are developed both humoral and cellular immune protection.</p>