Вирощування садивного матеріалу Salix L. in vitro

Abstract

Today’s alternative to traditional methods of plant reproduction is a cultivation in terms of in vitro, which enables to obtain the required amount of genetically homogeneous well-groomed seedlings throughout the year regardless of the growing season. In the plantation cultivation of species of Salix L genus to obtain plant biomass on pellets, the development and improvement of methods of microclonal reproduction of species and cultivars of this genus are prospective and relevant. Therefore, the aim of the research is to develop the methods for storage and long-term maintenance in the in vitro collection and rapid reproduction in the industrial volumes of the planting stock of S. viminalis L. and ‘Inger’ (S. viminalis × S. triandra L.). Methods. The explants had been cultivated according to the generally accepted method in a nutritious and nonhormonal in vitro, using the medium of Murashige and Skoog in their own modifications with changes in the amount of benzylaminopurine, Fe-chelate and activated carbon. The analysis of the obtained results was carried out by statistical methods. Results. The obtained results showed that while insertion of the hybrid cultivars ‘Inger’ to in vitro S. viminalis, sterilization should be done by adding Plant Preservative Mixture decontaminant in vitro. Intensive root formation and maximum reproduction factor for S. viminalis are 4,4, and for its cultivar ‘Inger’ is 6,2 with the concentration of 0,2 mg/l benzylaminopurine. Based on the obtained results, modification changes to the medium of Murashige and Skoog’s in vitro are required in order to receive a healthy planting stock. At the stage of reproduction it is necessary to add 0,2 mg/l of benzylaminopurine, 1,0 mg/l of AgNO3 to in vitro, and increase the content of Fe-chelate to 41,67 mg/l. At the root formation stage it is necessary to add 1,0 mg/l of AgNO3 , 1,0 g/l of activated carbon and 0,2–0,5 mg/l of indolyl butyric acid. Conclusions. To sum up, it is possible to achieve the mass reproduction of S. viminalis and its hybrid ‘Inger’ for the energy plantations creation in short term by using the Plant Preservative Mixture decontaminant, the hormone benzylaminopurine and the modifying changes to the medium of Murashige and Skoog’s in vitro.