Особенности получения культур in vitro реликтовых лекарственных растений с высоким содержанием биологически активных веществ (Taxus Canadensis Marsh., Ginkgo Biloba, Sequoia sempervirens L., Dioscorea caucasia Lypsky)

Abstract

The effect of the biochemical status of plants on the viability of explants and obtaining a productive culture of medicinal plants in vitro, taking into account the use of various sterilizing agents and photoperiod, was studied. It was found that the applied lighting mode had a significant effect on the intensity of callus tissue formation, its consistency and color. As a rule, callus tissue was formed in those places where the localization of phenolic compounds was insignificant. When calluses are initiated on explants characterized by a high content of polyphenols, they are released into the nutrient medium, which further leads to inhibition of growth processes. The resulting callus cultures were characterized by a high content of polyphenols, which were localized in cell walls and vacuoles.