Abstract
The alveolar epithelium is a dynamic tissue, consisting of cells of types I and II, covering more than 99 % of the lung inner surface and actively responding to various endogenous and exogenous stimuli. The technology of alveolocyte isolation is presented, which consists in mechanical disaggregation of tissue with subsequent processing of the resulting explants with 0.25 % trypsin solution in combination with filtration of the cell suspension through pores with a diameter of 100 µm and 50 µm. In two-dimensional static culture viable actively dividing rounded cells and large alveolar epithelial cells with cuboid or polygonal morphology, producing surfactant proteins, were visualized.
Highlights
Ключевые слова: культуры клеток; альвеолоциты I и II типа; метод ферментативной дезагрегации; морфология; клеточный выход; жизнеспособность
Получение культур альвеолоцитов I и II типа для медико-экологических исследований
The technology of alveolocyte isolation is presented, which consists in mechanical disaggregation of tissue with subsequent processing of the resulting explants with 0.25 % trypsin solution in combination with filtration of the cell suspension through pores with a diameter of 100 μm and 50 μm
Summary
Ключевые слова: культуры клеток; альвеолоциты I и II типа; метод ферментативной дезагрегации; морфология; клеточный выход; жизнеспособность. Культура альвеолярных эпителиальных клеток является уникальной системой, позволяющей in vitro изучить функциональное состояние клеток в норме, при воздействии факторов окружающей среды, а также смоделировать течение различных патологических состояний. Получение жизнеспособных и функционально состоятельных культур альвеолярных эпителиоцитов является основой для разработки новых подходов в регенеративной медицине заболеваний легких [11].
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