Abstract

Evaluation of various proteolytic enzymes efficiency for disaggregation of lung tissue is carried out and an optimized method for alveolar epithelial cells isolation is presented. This method includes mechanical disaggregation of tissue followed by processing of explanations with 0.25% trypsin solution in combination with filtration of the cell suspension through pores with a diameter of 100 gm and 50 gm. The obtained cell cultures were characterized by high viability (more than 91%) and morphological heterogeneity. Along with actively dividing rounded cells, differentiated alveolar epithelial cells with cuboid or polygonal morphology, characterized by high secretory activity, were visualized.

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