Магнитомеханическая стимуляция регенерации костной ткани

Abstract

The aim of the research. To assess the ability of superparamagnetic nanoparticles functionalized with the RGD peptide to accelerate bone tissue recovery aft er transverse osteotomy under the infl uence of a low-frequency alternating magnetic fi eld. Material and methods. We used superparamagnetic nanoparticles ferroarabinogalactans (FeAG) functionalized with the RGD peptide for targeted binding to integrins. Th e model for assessing the ability of FeAG-RGD to accelerate bone regeneration was the transverse osteotomy of the radius of the ICR mice. To induce the process of bone tissue regeneration aft er the injection of FeAG-RGD particles, mice were placed in an alternating magnetic fi eld (50 Hz 100 Oe) for 60 min. Th e therapy was performed daily for 5 days. Results. Th e paper tested the hypothesis about the acceleration of bone tissue regeneration by the method of magnetomechanical therapy using superparamagnetic FeAG-RGD nanoparticles. It has been established that FeAG-RGD under conditions of an alternating magnetic fi eld (50 Hz 100 Oe) are able to change their orientation in space and, thus, cause mechanical stress in the proteins of the cell membrane – integrins, which trigger the process of osteogenic diff erentiation. Th e research results showed that 5 procedures of magnetomechanical therapy lead to complete restoration of bone tissue at the site of the transverse osteotomy, while in the group of control animals no fusion of bones is observed. Conclusion. Th e use of the method of magnetomechanical therapy using ferroarabinogalactans functionalized with the targeting ligand RGD for binding to integrins of osteogenic cells is a promising technology for bone tissue regeneration