ГЕНЕТИЧЕСКИЙ СКРИНИНГ ПЕРСПЕКТИВНЫХ ГИБРИДОВ КАРТОФЕЛЯ РЕСПУБЛИКИ КОМИ

Abstract

The studies were conducted with the aim of genetic screening of promising selection hybrids of potatoes to identify genotypes resistant to a complex of phytopathogens. The study was carried out at A.V.Zhuravskiy Institute of Agrobiotechnology of Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences and Sintol company (Moscow) using the GenExpert reagent kit “Markers of potato resistance genes” (“Syntol”). For the study, 10 markers of genes encoding resistance to potato cancer, golden potato cyst nematode, pale potato cyst nematode, X-virus and Y-virus were selected (genes H1, Gro1-4 were detected using markers TG-689, 57R, N195 and Gro1-4-1, gene Sen1 – using marker NL25, gene Gpa2 – Gpa2-2). The objects of the study were leaves of plants of five potato hybrids undergoing selection testing in breeding nurseries: 2341-265 (Amur × Gala), 2339-8 (Oksania × Gala), 2339-9 (Oksania × Gala), 1992-14 (Udacha × Elmundo), 2000-60 (Colette × FZ 1867). The maximum number of markers (five in each) linked to resistance genes was revealed in samples 2339-8 and 1992-14: TG-689, 57R, N195 and NL25 in both hybrids; Gro1-4-1 marker in 2339-8 and Gpa2-2 in 1992-14. Only the marker of potato wart resistance gene, NL25, was noted in selection number 2341-265. Sample 2339-8 was characterized as a valuable source of R-genes, having in its genome the genes of resistance to golden potato cyst nematode (H1, Gro1-4) and potato wart (Sen1). The genotype of the 1992-14 hybrid, carrying the Gpa2 gene, is recommended for breeding new potato varieties resistant to various types of nematodes.