소루쟁이 뿌리 추출물의 배아 독성 및 항염 효능 평가

Abstract

In this study, a toxicity evaluation was conducted and anti-inflammatory effects were confirmed through in vitro and in vivo experiments with Rumex crispus L. Specifically, MTT, NO assay, iNOS, and COX-2 expression inhibition experiments were conducted using RAW 264.7 cells to confirm the in vitro toxicity and anti-inflammatory effectiveness of Rumex crispus L. In the in vivo toxicity assessment, coagulation rate, hatching rate, and heart rate were evaluated using zebrafish embryos and larvae. Results of the toxicity assessment, indicated that Rumex crispus L. root extract did not show toxicity at concentrations below 0.25mg/mL in RAW 264.7 cells. The NO assay confirmed that, iNOS, and COX-2 were inhibited in a concentrationdependent manner. In zebrafish embryos, an experimental animal model, it was confirmed that they did not coagulate at a concentration of 0.001 mg/ mL and that no hatching delay occurred. Therefore, the results of this study suggest that the root extract of Rumex crispus L. can be used as a cosmetic material that can be expected to have anti-inflammatory effects.