КОМПОЗИЦІЇ НА ОСНОВІ КОЛАГЕНУ З ГІДРОБІОНТІВ ЯК ПОТЕНЦІЙНІ РАНОЗАГОЮВАЛЬНІ ЗАСОБИ

Abstract

B a c k g r o u n d. The work was devoted to the study of compositions based on collagen extracted from hydrobionts as potential wound healing agents. The scales of Antarctic fish Champsocephalus gunnari, Nototheniidae and the biomass of jellyfish Diplulmaris antarctica were used as raw materials for obtaining collagen. M e t h o d s. To obtain collagen, the following steps were done: salting out non-collagen proteins with 10% NaCl, demineralization with 0.4 M HClO4, extraction of collagen with 0.5 M CH3COOH, reprecipitation of the obtained collagen with NaCl and CH3COOH, and lyophilization. R e s u l t s. Obtained collagen was collagen type I, which was confirmed by an electrophoretic analysis, in particular, the molecular weight of collagen chains (117 and 110 kDa) and their ratio as 2:1. The wound-healing effect of compositions based on 5% collagen was studied on a model of full-thickness wound in rats. The collagen compositions were prepared on 0.5% carbopol. Application of the compositions began on the third day after modeling the full-thickness wound; the wounds were treated every other day and until complete healing. C o n c l u s I o n s. A wound-healing effect of collagen-based compositions has been established, manifested in the acceleration of the wound-healing process. Thus, when applying compositions based on collagen from scales and collagen extracted from jellyfish to the wound surface, complete epithelialization of wounds was observed on the 18th day compared to the result in the group of animals where the wounds healed naturally and for which complete healing took place on the 22nd day.Although the composition based on fish scale collagen was more effective in the first week, an assessment of the wound area at the time of complete healing indicates a slightly better wound healing effect of the D. antarctica collagen-based composition.