ОЦЕНКА ТОКСИЧЕСКОГО ДЕЙСТВИЯ ИНСЕКТИЦИДА БОВЕРИКС, Ж НА МЕДОНОСНЫХ ПЧЕЛ А. MELLIFERA MELLIFERA L.

Abstract

The article presents the results of studies on the evaluation of the toxic effect of the biological insecticide Boveriks, G on honey bees A. mellifera mellifera L. Boveriks, G – bioinsecticide developed by research and development enterprise "BashIncom". It includes the entomopathogenic fungus Beaveria bassiana, strain VKPM F-1357. Biological fungicide BioSleep BW was used as a reference substance. The experiment used honey bees Apis mellifera mellifera L. of the Carpathian breed, which were kept in wooden cages. Acute oral toxicity was studied by feeding sugar syrup to bees with the addition of insecticides at concentrations of 10, 50 and 100 ml per 1 liter of syrup. For the test for acute contact toxicity, the anesthetized bees of the experimental groups were treated individually by applying 1 µl of an aqueous solution of insecticides in the same concentrations with a microapplicator to the upper part of the thorax of each bee. At the end of the experiment, 96 hours after feeding the bees with sugar syrup containing the insecticide Boveriks, G in different dosages, the mortality rate of bees in the experimental groups did not reach the level of 50 %. The maximum mortality rate was 16.7 %. There were no significant differences with the control and with the reference substance in terms of mortality in the experimental groups. The analysis of the data obtained allows us to conclude that the studied doses of the bioinsecticide Boveriks, G are not toxic to bees both during oral and contact exposure of the drug. The lethal dose LD50 in determining the acute oral toxicity of the active substance of the drug lies above the value of 1 × 105 CFU/bee; lethal concentration of LK50 – above the value of 100 ml/l, when determining acute contact toxicity LD50 – above the value of 1 × 104 CFU / bee; LK50 – above 100 ml/l.