Сучасні методи дослідження гострої токсичності полютантів для представників водної і наземної екосистем та впровадження їх у практику наукового центру превентивної та регуляторної токсикології

Abstract

The paper provides a brief historical background on the formation and development of ecotoxicology as a science, reflects the main modern methods and principles for studying the acute toxicity of xenobiotics for representatives of aquatic and terrestrial ecosystems recommended by the OECD (Organization for Economic Cooperation and Development). The classification of acute ecotoxicity of chemicals for land and water test systems is given. Further perspective development of the unit, which will be aimed at expanding ecotoxicological research in accordance with international OECD methods and GLP requirements, has been outlined. Aim. Revealing the main methods and principles recommended by the OECD for determining acute toxicity for biological objects of aquatic and terrestrial ecosystems and putting these methods and principles into practice of the Scientific Centre. Materials and Methods. The work was based on the implementation of international OECD standards and GLP principles. The OECD public electronic library of methods, particularly section 2 of the Instructions for Testing Chemicals, was used and analysed. Results and Conclusions. International methods for determining the acute toxicity of pollutants for non-target environmental objects were put into practice in the Scientific Centre, such as OECD 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test); OECD 202 (Daphnia sp. Acute Immobilisation Test); OECD 203 (Fish, Acute Toxicity Test); OECD 207 (Earthworm, Acute Toxicity Tests); OECD 213 (Honeybees, Acute Oral Toxicity Test); OECD 216 (Soil Microorganisms: Nitrogen Transformation Test); OECD 217 (Soil Microorganisms: Carbon Transformation Test); OECD 223 (Avian Acute Oral Toxicity Test). Systems for the quality and control of ecotoxicological studies have been developed, which made it possible to obtain national and international accreditation for the conduct of the above studies. Further activities of the department will be aimed at expanding ecotoxicological research using international OECD methods and compliance with GLP requirements, which will contribute to the development of ecotoxicological research both in the Scientific Centre and in Ukraine as a whole. Key Words: ecotoxicology, pesticides and agrochemicals, biological test systems, OECD methods, good laboratory practice (GLP).