ОТЕЧЕСТВЕННЫЕ ИЗОЛЯТЫ ВИРУСА АФРИКАНСКОЙ ЧУМЫ СВИНЕЙ В ФИЛОГЕНЕТИЧЕСКОМАНАЛИЗЕ ПО ГЕНУ B602L

Abstract

African swine fever virus has its own genetic mechanisms of variability. Of great interest to the scientific community is the problem of the evolutionary variability of the ASF virus genome. Namely, the problem of the evolutionary variability of the ASF virus genome, the study of the functions of individual genes, their role in interaction with the host cell and the impact on the evolutionary variability of both the virus itself and the susceptible organism. The diversity of genetic variants of the virus is the main property of this pathogen, as well as the lack of a vaccine against this disease. Having data on the genetic features of the ASF virus, as well as the study of the variability of known genes, allow us to expand information about the nature of genetic changes, as well as to obtain a molecular epizootological picture of the circulation of the ASF virus both in the Russian Federation and in the world as a whole. Analysis of a number of marker genes will make it possible to comprehensively assess the genetic changes in the ASF virus and their possible phenotypic manifestations. The study of the B602L gene allows more accurate differentiation of European and Asian isolates within the same genotype. Phylogenetic analysis for the B602L gene of sixty domestic isolates of the ASF virus isolated in the Russian Federation from 2016 to 2017 was carried out. As a result of the study, the isolates used in the work were divided into two clusters. Analysis of the obtained data showed the high conservation of isolates and strains and made it possible to establish their genotypic affiliation. The nature of genetic and phenotypic changes in the genome of the ASF virus still remains unclear.