Abstract
African Swine Fever Virus, Tanzania, 2010–2012
Highlights
In October 2011, the disease spread to the Sumbawanga District of the Rukwa region through feeding of swill and illegal movement of animals
End of the B646L (p72) gene was amplified by using p72U/p72D primers [2] and subjected to automated dideoxynucleotide cycle sequencing by using Big Dye Terminator Cycle Sequencing Kit Version 3.1 (Applied Biosystems, Foster City, CA, USA) in a 24-capillary DNA sequencer (Genetic Analyzer 3500 xL; Applied Biosystems)
The Georgia 2007/1 African swine fever virus (ASFV) isolate was detected in Georgia in 2007 and has caused African swine fever (ASF) outbreaks in Armenia, Azerbaijan, and Russia [5]
Summary
In October 2011, the disease spread to the Sumbawanga District of the Rukwa region through feeding of swill and illegal movement of animals. DNA was extracted from spleens of animals that either died of the disease or were killed at slaughterhouses during 2010–2012. End of the B646L (p72) gene was amplified by using p72U/p72D primers [2] and subjected to automated dideoxynucleotide cycle sequencing by using Big Dye Terminator Cycle Sequencing Kit Version 3.1 (Applied Biosystems, Foster City, CA, USA) in a 24-capillary DNA sequencer (Genetic Analyzer 3500 xL; Applied Biosystems).
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