ЛАБОРАТОРНА ХАРАКТЕРИСТИКА РІДКОГО ЕНЗИМНОГО МИЙНОГО ЗАСОБУ «ЕНЗИМИЙ» ДЛЯ САНОБРОБКИ У МОЛОЧНІЙ ПРОМИСЛОВОСТІ

Abstract

We have developed a liquid enzyme detergent «Enzymyi» for sanitization of CIP–installations on milk plants that includes potassium hydroxide, complexones, proteolytic enzyme, stabilizers, and distilled water.The previous laboratory studies showed that the peak activity of «Enzymyi» on milk proteins was observed at as high as 65.8% at 60°C. Its activity decrease with the rise of temperature above 60 °C due to denaturation of enzymes since the heat causes changes in the primary structure and conformation of enzyme. It is also established that at pH solutions of 8.35 units proteolytic activity increased 1.7 times (P ≤ 0.01) compared to 7.2 pH units and reached its maximum activity 65.8 – 64%. Further increase of pH solutions from 8.35 units to 8.6 units contributed to gradual inhibition of enzymes resulting in reduction of proteolytic activity to 0. Proteolytic activity of the studied sample of enzyme detergent at water hardness of 0.357 mg–eq/l also reached the maximum of 60.5% and at increasing water hardness from 0.714 to 1.071 mg– eq/l PA decreased by 25.4% (P ≤ 0,05), i.e. with increasing water hardness PA reduced respectively. The article presents the study results as regards foam generating ability, foam stability and surface tension of solutions. The liquid detergent enzyme «Enzymyi» in concentrations of 0.03; 0.05; 0.07; 0.1; 0.3; 0.5; 0.7; 1% at the temperatures 20 °C and 60 °C was used for study. The article presents the study results as regards foam generating ability, foam stability and surface tension of solutions. The liquid detergent enzyme «Enzymyi» in concentrations of 0.03; 0.05; 0.07; 0.1; 0.3; 0.5; 0.7; 1% at the temperatures 20 °C and 60 °C was used for study.The results showed that «Enzymyi» practically doesn’t develop foam generating ability at the concentration from 0.03 to 1%. Foam stability does not exceed the standard, thus no more than 50% of the solution in 5 minutes that allows using the available composition for circulation washing of dairy equipment without its dismounting.