Abstract
The use of detergents with enzymatic activity is very popular if we consider their real economic advantages - reducing energy and water consumption but also reducing the environmental impact by using more biodegradable components related to organic and inorganic components. The purpose of our study was to evaluate the impact of environmental factors such as the variability of water hardness in the public water supply network and the temperature applied to the amylolytic, lipolytic and proteolytic enzyme activity of 6 detergents available on the market. The determinations were performed by modified methods from the European Pharmacopoeia 8th Edition in determination the enzymatic activity of the pancreas powder. The determinations show a significant influence of increased water hardness on lipase and protease activity in detergent (p [0.01 *) between extreme hardness values, while amylolytic activity does not vary significantly with increasing water hardness.
Highlights
Water quality is extremely important for enzymatic activity, as it is a type of catalysis that occurs in an aqueous environment
Amylases, lipases and cellulases are enzymes commonly used in detergents to improve their washing properties to reduce the cost of this process - reducing the temperature of the washing process, water consumption and, at the same time, the impact on the environment reducing the concentration of potentially toxic ingredients on the environment [2]
Scientific literature offers a variety of studies with the aim to improve the detergent washing properties and to change their composition to be ecofriendly - reducing the amount of additives used - surfactants, phosphates, their replacement with bio-compounds - enzymes, and the influence of these additives on the enzymatic activity [5], there are studies on the influence of water hardness on the efficacy of surfactants sodium oleate, linear dodecylbenzene sulfonate or sodium dodecyl sulfate [6, 7], but there are no studies on the modification of the proteolytic, amylolytic and lipolytic enzymatic activity in detergents, depending on the hardness of the water, during a washing cycle in the washing machine
Summary
Nwaa2sSd2Oe3t.eTrmheinaecdtiivnitythoef a standard pancreatin suspension conditions described above. Determination of the enzymatic activity of detergents directly into samples of dUif1fUerletrnatThhaerrdmnoesstsataicndWtahteercBaastehinwsituhbwstraateter was performed by methods described in European was added. Pharmacopoeia 8th Edition for pancreatin powder and Determination of the lipolytic activity of detergents was adapted for detergent determinations [15]. The standard substance was prepared from 150 mg of sample with the substrate (Emulsio Oleosa, Romanian pancreatin powder (10,000 units of lipase, 8,000 units Pharmacopoeia 10th Edition) for 30 min at 20°C and titrating amylase, 600 units of protease activity quantified by Ph the free fatty acids with 0.05N NaOH solution in the. The detergent was water so that it was diluted 50 times. The detergent sample was diluted 100 times as dinitlurotedducwedithinwtoatUe1r. After incubation 960 ethanol was added to inhibit enzyme activity and samples were obtained and the acidity was
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