Abstract
Objectives. To evaluate the diagnostic efficacy of the prick-test and the levels of tryptase, cations K<sup>+</sup>, NH<sub>4</sub><sup>+</sup> and Ca<sup>2+</sup>, peroxidase activity in the oral fluid (OF) as biomarkers of apple allergy and to comparatively assess them. Material and methods. We examined 35 patients with a history of allergic reactions to a fresh apple (the study group) and 29 healthy persons (the control group). All participants underwent the prick-test with 3 varieties of fresh apples and oral-pharyngeal provocative test (OPPT) with freshly prepared apple juice with the assessment of tryptase, peroxidase activity, K<sup>+</sup>, NH<sub>4</sub><sup>+</sup> and Ca<sup>2+</sup> cations levels in the OF. Results. The prick-test was positive in 94% of patients. In 70% of patients after OPPT, there was a significant tryptase level increase in the OF compared with healthy persons (p=0.000003). After provocation, in the study group, in 74% of cases, a significant peroxidase activity level increase in the OF was revealed in comparison with the control group (p<0.0000001). A significant K<sup>+</sup> level increase in the oral fluid was found in 81% of patients, in contrast to healthy persons after OPPT (p=0.02). In 67% of cases in the study group, a significant NH<sub>4</sub><sup>+</sup> level decrease after OPPT was found in comparison with the initial cation level in this group (p=0.039). Patients with a history of apple allergy after provocation showed a higher Ca<sup>2+</sup> cations level in the OF than the control (p=0.02). Conclusions. Biomarkers of hypersensitivity to apples of the varieties Golden Delicious, Red Prince and Belarusian sweet were revealed: positive prick-test, an increase of tryptase, myeloperoxidase activity, K<sup>+</sup> cations and a decrease of NH<sub>4</sub><sup>+</sup> level after OPPT. Biomarkers have a high diagnostic value and can be used both in combination and separately to identify systemic and local hypersensitivity and to diagnose food allergy to apples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
