Abstract
The spectrum of soluble proteins of spring wheat leaves was studied for foliar treatment with chelated copper, molybdenum-containing microfertilizer ZhUSS-2 (0.1% solution). Electrophoretically, polypeptides with a molecular mass of 13.2 - 145 kD were detected. The qualitative composition of soluble proteins in the leaves of spring wheat plants under the influence of foliar treatment during the vegetation did not change, however, their quantitative changes were observed. During a single treatment of spring wheat in the tillering phase, an increase in polypeptides with a molecular mass of 145 and 94 kD was recorded; the content of polypeptides with molecular weights of 13.2 - 66 kD decreased in comparison with the control sample. When the plants were sprayed twice in the tillering and tubing phases, the content of these polypeptides as well as polypeptides with molecular weights of 45 and 41 kD increased. The content of other detected polypeptides also decreased compared to the control variant. During a three-fold treatment of spring wheat plants in the tillering, tubing and blossoming-flowering phases with the ZhUSS-2 preparation, the number of all detected polypeptides increased in comparison with the control sample. Thus, the response of the metabolism of soluble proteins in the cells of spring wheat leaves to the action of foliar top dressing with copper, molybdenum-containing chelate microfertilizer of ZhUSS-2 brand in different phases of vegetation is different and depends on the dose of exposure. Particular attention should be paid to the role of two polypeptides in the treatment of vegetative plants by ZhUSS-2 M.M. 94 kD and, mainly, 145 kD. There is a reason to believe that copper molybdenum-containing chelate microfertilizer ZhUSS-2 exhibits the action of an abiogenic elicitor, which leads to activation of the gene apparatus of cells. The possible functional role of these proteins is discussed.
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