Опыт использования гамма-облучения для стерилизации композиционного материала «Углекон-МЯ», применяемого в медицине

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The carbon-composite material is actively used in medicine. In particular there is a successful practice of its use for replacing bone defects in orthopedics, traumatology and maxillofacial surgery. Meanwhile the cellular structure of the material and the presence of a large number of closed pores impose certain requirements on the process of its disinfection. The radiation method of sterilization of inorganic implants, as well as biotransplants, has recently become increasingly widespread in medicine. The purpose of this study was to evaluate the effectiveness of various doses of radiation for the sterilization of high-porous carbon material “Ugleсon-M”. Twelve samples of this material were infected with strains of various microorganisms and divided into 4 batches depending on the planned dose of radiation. Each batch was exposed to radiation doses 25, 50; 75 and 100 kGy respectively. The control sample was not irradiated. After the radiation exposure each sample, including the control one, was washed and inoculated on special liquid nutrient medium followed by seven-days incubation and daily inoculation on solid nutrient medium. All doses of ionizing radiation proposed in the study had a microbicidal effect, ensuring the sterility of the studied material. On the control sample the microorganisms remained viable. Experimental data on radiation sterilization of carbon material are presented for the first time. It has been established that all studied radiation doses, including the minimal one (25 kGy), cause the death of not only vegetative forms of microorganisms (bacteria, fungi), but also spores. A conclusion is made about the possibility of using radiation sterilization for highly porous carbon-composite material.