Значення порушень прооксидантно- антиоксидантних процесів у патогенезі розвитку експериментальної пневмонії та адреналінового пошкодження міокарда

Abstract

Today, pneumonia is one of the most common diseases of the respiratory system, accounting for 35-45%. No less common is coronary heart disease (CHD) among the pathologies of the cardiovascular system. In the medical world, the problem of comorbid pathology is acute, which can reduce adaptive capabilities, reduce the effectiveness of treatment and increase the development of various complications. Pathogeny of development of pneumonia and CHD is in this time to the end not studied. Research aim: to find out the features of changes and role of lipoperoxidation processes and state of the antioxidant system in pathogenesis of development of experimental pneumonia of connected with the adrenalin damage of myocardium. Materials and research methods. There were undertaken studies on 82 guinea-pigs (males), that distributed on three groups: the first group is intact animals – control (10); the second (experience) group is animals with experimental pneumonia (ЕP) accordingly on 1th, 3th, 6th and 14th twenty-four hours of experiment (36); the third (experience) group is animals with the united pathology (ЕP and adrenaline myocardium damage – AMD) accordingly on 1th, 3th, 6th and 14th twenty-four hours of experiment (36). The fixed twenty-four (1th, 3th, 6th and 14th twenty-four hours) hours took into account, because they answer the classic stages of the abrupt used for setting fire reply (incubations, development of illness, height of illness, convalescence) and motion. ЕP recreated by the method of Shlapnikov V. N., Solodova T. L. et al. by the way of intranasal infection of animals of Staphylococcus of aureus. AMD designed by non-permanent intramuscular introduction of 0,18% solution adrenaline hydrotartrate («Darnytsia», Ukraine), in a dose 0,5 mg/kg. Assessment of lipid peroxidation was carried out by measuring levels of diene conjugates (DC) and malonyldialdehyde (MDA), and activity of antioxidant system was evalution by catalase (CAT) and superoxide dismutase (SОD). Euthanasia by decapitation of laboratory animals was conducted under act of chloroform anaesthesia. Determinations of DC in fabrics of kidney was carred by Gavrilov's method, content of МDА – by the method of Y. N. Korobeynikov, activity SОD – by the method of R. Fried, CAТ – by the method of B. Holmes and C. Masters. The statistical working of the obtained data was carried out by Student's t-test. Results and discussions. ЕP and AMD (1th, 3th, 6th, 14th twenty-four hours) is accompanied by the increase of content of DC in fabrics of kidneys accordingly on 62,5 %, 71,5 %, 79,5 %, 82,9 % (р < 0,05), MDA – on 55,0 %, 62,1 %, 72,7 %, 85,7 % (р < 0,05) on a background the decline of activity of SОD accordingly on 25,2 %, 27,2 %, 30,6 %, 37,2 % (р < 0,05), CAT – on 31,2 %, 34,7 %, 40,3 % 45,5 % (р < 0,05) in relation to the control group of animals, that testified to development of oxidative stress, that strengthened motion of pneumonia and AMD. Conclusion: the results of researches are Got by us allow to assert that comorbidity (EP and AMD) predetermines violation of balance between prooxidant and by an antioxidant the systems, strengthening of lipoperoxidation processes in the conditions of depression of the antioxidant securing for all stages of their development with advantage on 6th and 14th twenty-four hours of experiment against the intact group of animals and specifies on the important role of one of molecular (lipid) mechanisms of damage of cages in pathogenesis of formation of these models of illnesses.